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Tuesday 26 March 2019

DNA Extraction

Aim

To extract DNA from a kiwifruit

Method

1. Using a spatula, scoop 1/2 kiwifruit into a Ziploc bag. Add 25mls of water to the bag along with a decent pinch of salt.
2. Mush it into a thick soup. Pour over a cloth, into a funnel and then into a beaker.
3. Pour liquid into a a test tube, add a squirt of dishwashing liquid, swirl together and the leave to sit for five minutes
4. After allowing to sit, tilt the test tube at a 45° angle, and the slowly pour 10mls of ethanol down the side.
5. Let sit.

Results
It worked. The DNA of the kiwifruit floated up to the top and separated itself from the other stuff that we put in the test tube. The water helped us break up the kiwifruit, and the salt helped clump up the strands of DNA. Then the ethanol helped us see the actual strands of DNA. It smelt like lime soda, I don't understand why but it did and I'm very still confused about how a kiwifruit smelt like it was lime.

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